Description
The 3M Attest Super Rapid Readout Biological Indicator 1491 is a self-contained biological indicator specifically designed for rapid and reliable qualification testing and routine monitoring of 270° F (132° C) and 275° F (135° C) gravity-displacement steam sterilization processes when used in conjunction with the 3M Attest Auto-reader 490, a 3M Attest Auto-reader 490H having software version 4.0.0 or greater or a 3M Attest Mini Auto-reader 490M. The 1491 biological indicator is a single-use device. The self-contained design includes a spore carrier with spores of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) and a media ampoule containing bacteriological media which meets the requirements for growth promoting ability. The spore carrier and media ampoule are contained in a plastic vial topped with a blue cap. A chemical process indicator which changes from pink to light brown or darker upon exposure to steam is located on the top of the cap. The 1491 biological indicator utilizes the âºâ€‘glucosidase enzyme system, which is generated naturally within growing cells of Geobacillus stearothermophilus. The âºâ€‘glucosidase in its active state is detected by measuring the fluorescence produced by the enzymatic hydrolysis of a non-fluorescent substrate, 4-methylumbelliferyl‑âºâ€‘D-glucoside (MUG). The resultant fluorescent by-product, 4-methylumbelliferone (MU) is detected in the 490 Auto-reader. The presence of fluorescence within the specified incubation time of the 1491 biological indicator in the 490, 490H, or 490M Auto-reader indicates a steam sterilization process failure.
